High-risk Human Papillomavirus Determination in Formalin-fixed, Paraffin-embedded Cervical Tissue Using the Roche Cobas 4800 System: A Comparative Study With Liquid-based Cytology.

Roche cobas 4800 human papillomavirus (HPV) test is an automated real-time polymerase chain reaction-based system that allows the simultaneous detection of 14 human papillomavirus high-risk (HR-HPV) genotypes. This test is Food and Drug Administration approved since 2011 for HPV determination in liquid-based cytologic samples, but a clinically validated technique for formalin-fixed, paraffin-embedded (FFPE) tissue specimens is presently not commercially available. In our laboratory, we have developed an HPV detection procedure in FFPE tissue by cobas 4800 HPV test. In order to validate our method, we retrospectively studied 165 FFPE cervical biopsy and conization specimens with varied diagnoses from our files. In 50 of them, we contrasted the results with those obtained from simultaneous liquid-based cytologies from the same patients. Finally, seeking the possible complementary clinical usefulness of the procedure, we compared the HPV genotypes detected in cervical intraepithelial neoplasia grade 1 (CIN1)-diagnosed biopsies from 20 patients with a subsequent high-grade CIN (CIN2+) diagnosis with those from another group of 20 patients without a subsequent CIN2+ diagnosis. Eighty-seven percent of the assays provided informative results. HR-HPV was detected in 28 of 32 (88%) invasive cervical squamous carcinomas. Coincidental HR-HPV genotypes were obtained in 32 of 50 (64%) cases with simultaneous cervical biopsy and liquid-based cytologic samples. A significant higher risk of progression to CIN2+ was found when HPV16 (P=0.022) or any HR-HPV genotype (P=0.037) was detected in CIN1 biopsies. The reported procedure provides an automated, technically time-saving, easy to integrate into laboratory routine, and reliable method of HR-HPV determination in FFPE specimens.

Distribution of the Peruvian Plantcutter Phytotoma raimondii (Passeriformes: Cotingidae)

The Peruvian Plantcutter, Phytotoma raimondii Taczanowski, 1883, is a restricted-range species endemic to coastal northern Peru. Historically its range is given from Tumbes in the extreme north-western Peru, south to the northern part of Lima Department. Although an increasing amount of information on the Peruvian Plantcutter exists, from historical records to new locations, it has remained dispersed, sometimes unverified, not systematized, and largely unpublished. A careful revision of museum collections as well as published and unpublished records results in a total of 53 sites where the species has been recorded and that represent the present knowledge of the distribution of the species.

La cortarrama peruana, Phytotoma raimondii Taczanowski, 1883, es una especie endémica y de distribución restringida a la costa norte del Perú. Históricamente su rango ha sido considerado desde Tumbes en el extremo noroeste del Perú y hacia el sur hasta la parte norte del Departamento de Lima. Aunque existe mayor cantidad de información sobre la cortarrama peruana, entre registros históricos y localidades nuevas, esta información ha permanecido dispersa, a veces no verificada, no sistematizada y consecuentemente no publicada. Una revisión meticulosa de ejemplares de museos así como de registros publicados y no publicados da como resultado un total de 53 localidades donde la especie ha sido registrada y que representan el conocimiento actual de su distribución.

Evaluacion de aves en la laguna El Paraiso, Lima, Perú / Bird evaluation at El Paraíso lagoon, Lima, Peru

Este estudio se realizó en la laguna El Paraíso, departamento de Lima, Perú, de abril de 1999 a abril del 2000. Tuvo como objetivos la determinación de la diversidad específica en aves; la densidad poblacional de las principales familias de uso cinegético y la determinación de los microhábitats más importantes para los grupos evaluados. Se reportaron 81 especies de aves las cuales pertenecen a 62 géneros y a 35 familias. En esta cifra se reportan 19 especies nuevas para el área de Paraíso; el 46% de la diversidad de aves está dada por las familias Scolopacidae (14%), Ardeidae (10%), Laridae (7%), Anatidae (5%), Charadriidae (5%) y Columbidae (5%). La familia Rallidae es la más abundante y está representada por dos especies Gallinula chloropus "polla de agua" y Fulica ardesiaca "gallareta", siendo la primera más numerosa. Asimismo, se dan las recomendaciones para la conservación del área.

This study was carried out at El Paraíso lagoon, Lima, Peru, from April 1999 to April 2000. The main goals was determinated the birds specific diversity, the population density of main cinegetic bird families and the determination of the most important microhabitats for the studied groups. 81 birds species (19 species are new for the area) were reported, distribuited in 62 generas and 35 families. 46% of the diversity is given for the following families: Scolopacidae (14%), Ardeidae (10%), Laridae (7%), Anatidae (5%), Charadriidae (5%) and Columbidae (5%). The Rallidae family is the most abundant and is represented for two species Gallinula chloropus "Common Moorhen" y Fulica ardesiaca "Slate-colored Coot". It is given recommendations for the conservation of the area.